MHC Class II I-Ap antibody

Catalog No. ABIN114314

The Mouse Monoclonal anti- antibody has been validated for FACS and CyTox. It is suitable to detect in samples from Mouse.

Quick Overview for MHC Class II I-Ap antibody (ABIN114314)

Target: MHC Class II I-Ap

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Cytotoxicity Test (CyTox)

Clone: 7-16-17

Product Details

Purification: Protein G chromatography

Immunogen: B10.p

Isotype: IgG2a

Application Details

Application Notes: Flow cytometry (protocol see below). Cytotoxic assay.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1x10e6 cells, representing 1 test). 4. To each tube, add 0. 2-1. 0 µg of antibody. 5. Vortex the tubes to ensure thorough mixing of antibody and cells.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS and 0.02 % sodium azide (NaN3).

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody (undiluted) at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details

Target: MHC Class II I-Ap